Typhoid fever caused by Salmonella typhi recombinant is an endemic disease in Asia, including Indonesia. According to data from the World Health Organization (WHO), there are 81% of every 100,000 people in Indonesia infected with typhoid fever. Based on this, the development of a typhoid fever detection kit that is simple, rapid, specific and sensitive is still needed. One of the currently available detection kit developments is the antigen-antibody interaction approach.
This study aims to obtain complete information on the purification of recombinant Fim-C-S. typhi proteins as antigens for raw materials for the manufacture of typhoid fever detection kits. This study uses the IMAC method with Ni2+ metal, which has advantages in high selectivity and purity. The results of this study report the purification of recombinant Fim-C-S. Typhi protein inclusion bodies with variations in the 3-, 5-, and 7-fold binding and washing process gave corresponding percentage yields of 37.84, 39.44, and 38.21% pure protein.
Previous studies also reported variations in binding and washout at 2-, 4-, and 6-fold and gave percentage yields of 35.37, 39.11, and 40.49%, respectively. Based on the data, we conclude that the 6-fold variation is the best repeat variation to obtain the highest percentage yield so that it can be used as a reference in large-scale purification.
Salmonella typhi uspA recombinant protein (His tag)
Purity: > 90% SDS-PAGE.
Expression system: Mammal
Protein length: full-length protein
Species: Salmonella typhi
Amino acids: 2 to 144
Tags: Your N-Terminus Tag
Additional sequence information: mature chain
Our Abpromise warranty covers the use of ab267923 in the following tested applications. Application notes include recommended starting dilutions; the end-user must determine the optimal dilutions/concentrations.
Applications: SDS PAGE
Concentration: 10 µg to 0.21 mg/mL
Stability and Storage
Shipped at 4°C. Store at +4°C for the short term (1-2 weeks). At the time of aliquot delivery. Store at -20°C or -80°C. Avoid freeze/thaw cycle.
- pH: 7.2
- Components: PBS, 6% trehalose