Specific Features of Intracellular Signal Transduction in the Regulation of Functions of Neural Stem Cells and Committed Neuronal Progenitors

Specific Features of Intracellular Signal Transduction in the Regulation of Functions of Neural Stem Cells and Committed Neuronal Progenitors

We studied the function of NF-κB-, cAMP/PKA-, JAKs/STAT3-, ERK1/2-, p38-, JNK- and p53- mediated signaling pathways within the realization of the expansion potential of neural stem cells and dedicated neuronal progenitors beneath in vitro situations. The tactic of pharmacological blockade with selective inhibitors of particular person signaling molecules revealed some principal variations of their function within the dedication of the proliferation and differentiation standing of progenitor cells of various lessons.

Evaluation of the peculiarities of intracellular signaling in cells and comparability of the function of its particular person components attest to the prospects of growing new medication with neuroregenerative exercise based mostly on STAT3 inhibitors or JNK activators. These modulations of exercise of signaling molecules can stimulate the conclusion of the expansion potential of dedicated neuronal progenitors and impartial stem cells, respectively. The blockade of STAT3 and a rise within the content material of phosphorylated types of JNK had no “detrimental” results on the functioning of multipotent neural stem cells and dedicated neuronal progenitors, respectively.

Porphyromonas gingivalis (P. gingivalis), a Gram-negative oral anaerobe, is taken into account to be a serious pathogenic agent concerned within the onset and development of persistent periodontitis. P. gingivalis should be capable of understand and reply to the sophisticated modifications in host to outlive the environmental challenges, through which the two-component sign transduction techniques (TCSs) play essential roles by connecting enter indicators to mobile physiological output. Canonical TCS consists of a sensor histidine kinase and a cognate response regulator that features through a phosphorylation cascade.

cAMP-regulated phosphoproteins DARPP-32, ARPP16/19, and RCS modulate striatal sign transduction via protein kinases and phosphatases

Many years of analysis led by Paul Greengard recognized protein phosphorylation as a ubiquitous and very important post-translational modification concerned in lots of neuronal signaling pathways. Specifically, his discovery that second messenger-regulated protein phosphorylation performs a central function within the propagation and transduction of indicators within the nervous system has been important in understanding the molecular mechanisms of neuronal communication. The institution of dopamine (DA) as a necessary neurotransmitter within the central nervous system, mixed with observations that DA prompts G-protein-coupled receptors to manage the manufacturing of cyclic adenosine monophosphate (cAMP) in postsynaptic neurons, has supplied elementary perception into the regulation of neurotransmission.

Notably, DA signaling within the striatum is concerned in lots of neurological features corresponding to management of locomotion, reward, habit, and studying, amongst others. This evaluation focuses on the historical past, characterization, and performance of cAMP-mediated regulation of serine/threonine protein phosphatases and their function in DA-mediated signaling in striatal neurons. A number of small, heat- and acid-stable proteins, together with DARPP-32, RCS, and ARPP-16/19, had been found by the Greengard laboratory to be regulated by DA- and cAMP signaling, and located to endure a posh however coordinated sequence of phosphorylation and dephosphorylation occasions.

These research have contributed considerably to the institution of protein phosphorylation as a ubiquitous and very important course of in sign propagation in neurons, paradigm shifting discoveries on the time. Understanding DA-mediated signaling within the context of sign propagation has led to quite a few insights into human situations and the event of therapies and therapies. Importantly, many research have discovered that mineral vitamin, corresponding to N, P, and Fe, not solely impacts fruit high quality straight but in addition influences the absorption and the content material of different nutrient components.

On this evaluation, we offer insights of the mineral vitamins into their operate, transport, sign transduction related to Rosaceae fruit high quality, and postharvest storage at physiological and molecular ranges. These research will contribute to offer theoretical foundation to enhance fertilizer environment friendly utilization and fruit trade sustainable growth. On this evaluation, the roles of TCSs in P. gingivalis had been demonstrated by illustrating the goal genes and modulation modes, which can assist elucidate the underlying mechanisms in future research.

Specific Features of Intracellular Signal Transduction in the Regulation of Functions of Neural Stem Cells and Committed Neuronal Progenitors

Deciphering cell-type particular sign transduction within the mind: Challenges and guarantees

Sign transduction designates the set of molecular occasions that happen inside a cell upon extracellular stimulation to mediate a useful final result. Many years after the invention that dopamine triggers opposing signaling pathways in D1- and D2-expressing medium spiny neurons, it’s now clear that there are as many alternative flavors of signaling pathways within the mind as there are neuron varieties. One of many largest challenges in molecular neuroscience is to elucidate cell-type particular signaling, so as to perceive neurological ailments with regional vulnerability, but in addition to establish targets for precision medication devoid of off-target results. Right here, we make a case for the significance of the research of neuron-type particular molecular traits.

We then evaluation the applied sciences that exist to check neurons of their full range and spotlight their disease-relevant idiosyncrasies. Mineral vitamin, taken up from the soil or foliar sprayed, performs elementary roles in plant development and growth. Amongst of no less than 14 mineral components, the macronutrients nitrogen (N), potassium (Okay), phosphorus (P), and calcium (Ca) and the micronutrient iron (Fe) are important to Rosaceae fruit yield and high quality. Deficiencies in minerals strongly have an effect on metabolism with subsequent impacts on the expansion and growth of fruit bushes.

Rat signal transduction-Smad1 ELISA Kit

CN-01425R1 96T
EUR 439

Rat signal transduction-Smad1 ELISA Kit

CN-01425R2 48T
EUR 290

Rat signal transduction-Smad7 ELISA Kit

CN-01426R1 96T
EUR 442

Rat signal transduction-Smad7 ELISA Kit

CN-01426R2 48T
EUR 293

Mouse signal transduction-Smad1 ELISA Kit

CN-02562M1 96T
EUR 480

Mouse signal transduction-Smad1 ELISA Kit

CN-02562M2 48T
EUR 331

Human signal transduction-Smad1 ELISA Kit

CN-03384H1 96T
EUR 448

Human signal transduction-Smad1 ELISA Kit

CN-03384H2 48T
EUR 297

Human signal transduction-Smad7 ELISA Kit

CN-03385H1 96T
EUR 448

Human signal transduction-Smad7 ELISA Kit

CN-03385H2 48T
EUR 297

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
EUR 1108
  • Category: Long Non-coding RNA Tools

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

Anti-ERK1 (E32) Signal Transduction Monoclonal Antibody

ABP-MAB-ES001 100 ug Ask for price
    • Product line: Tag Antibodies
    • Brand: Epitope Bio

Anti-ERK1 (E19) Signal Transduction Monoclonal Antibody

ABP-MAB-ES002 100 ug Ask for price
    • Product line: Tag Antibodies
    • Brand: Epitope Bio

Anti-ERK1/2 (E31R) Signal Transduction Monoclonal Antibody

ABP-MAB-ES003 100 ug Ask for price
    • Product line: Tag Antibodies
    • Brand: Epitope Bio

Mouse Signal transduction protein CBL- C, Cblc ELISA KIT

ELI-33118m 96 Tests
EUR 865

Human Signal transduction protein CBL- C, CBLC ELISA KIT

ELI-50273h 96 Tests
EUR 824

Bacillus subtilis Signal transduction histidine-protein kinase/phosphatase DegS (degS)

1-CSB-EP320275BRJ
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 49 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Bacillus subtilis Signal transduction histidine-protein kinase/phosphatase DegS(degS) expressed in E.coli

QuantiSir Specific Gene Knockdown Quantification Kit For Signal Transduction - ViDR

P-5008 96 Assays
EUR 788.05
Description: Ask the seller for details

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Antibody

20-abx128380
  • EUR 453.00
  • EUR 133.00
  • EUR 1302.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 2 (KHDRBS2) Antibody

abx034990-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 2 (KHDRBS2) Antibody

abx034990-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Antibody

20-abx104762
  • EUR 467.00
  • EUR 133.00
  • EUR 1344.00
  • EUR 634.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 2 (KHDRBS2) Antibody

20-abx007227
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 2 (KHDRBS2) Antibody

20-abx307678
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Antibody

20-abx327286
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 2 (KHDRBS2) Antibody

abx224066-100ug 100 ug
EUR 411
  • Shipped within 5-10 working days.

Recombinant KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1)

4-RPL650Hu01
  • EUR 499.62
  • EUR 236.00
  • EUR 1598.56
  • EUR 599.52
  • EUR 1099.04
  • EUR 397.00
  • EUR 3846.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q07666
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 28.9KDa
  • Isoelectric Point: Inquire
Description: Recombinant Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 expressed in: E.coli

Recombinant KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1)

4-RPL650Mu01
  • EUR 510.37
  • EUR 239.00
  • EUR 1638.88
  • EUR 612.96
  • EUR 1125.92
  • EUR 404.00
  • EUR 3947.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q60749
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 28.2kDa
  • Isoelectric Point: 9.5
Description: Recombinant Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 expressed in: E.coli

Array Designer

AD6 1
EUR 4248

Nylon Array

MVSNGA110 10 Slides
EUR 226

Nylon Array

MVSNGA120 20 Slides
EUR 363

Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Protein

20-abx067639
  • EUR 718.00
  • EUR 286.00
  • EUR 2207.00
  • EUR 843.00
  • EUR 509.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Protein

20-abx166241
  • EUR 704.00
  • EUR 286.00
  • EUR 2151.00
  • EUR 829.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Green qPCR SuperMix

20-abx098031
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
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Probe qPCR SuperMix

20-abx098040
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
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Universal qPCR Mixture

QPCR1000-UNIV 4X1.25ml
EUR 276.2
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human)

4-PAL650Hu01
  • EUR 261.00
  • EUR 2734.00
  • EUR 676.00
  • EUR 330.00
  • EUR 220.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Lys152~Glu381)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1)

Transduction Peptide 1 (alfa II)

5-02015 4 x 1mg Ask for price

TransDux virus transduction reagent (200x)

LV850A-1 80 transductions
EUR 313
  • Category: Lentiviral Technology

TransDux MAX Lentivirus Transduction Enhancer

LV860A-1 100 transductions
EUR 450
  • Category: Lentiviral Technology

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human), APC

4-PAL650Hu01-APC
  • EUR 367.00
  • EUR 3581.00
  • EUR 989.00
  • EUR 470.00
  • EUR 228.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Lys152~Glu381)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with APC.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human), Biotinylated

4-PAL650Hu01-Biotin
  • EUR 327.00
  • EUR 2684.00
  • EUR 783.00
  • EUR 403.00
  • EUR 225.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Lys152~Glu381)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with Biotin.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human), Cy3

4-PAL650Hu01-Cy3
  • EUR 447.00
  • EUR 4733.00
  • EUR 1277.00
  • EUR 585.00
  • EUR 263.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Lys152~Glu381)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with Cy3.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human), FITC

4-PAL650Hu01-FITC
  • EUR 313.00
  • EUR 2884.00
  • EUR 811.00
  • EUR 396.00
  • EUR 202.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Lys152~Glu381)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with FITC.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human), HRP

4-PAL650Hu01-HRP
  • EUR 334.00
  • EUR 3120.00
  • EUR 873.00
  • EUR 424.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Lys152~Glu381)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with HRP.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human), PE

4-PAL650Hu01-PE
  • EUR 313.00
  • EUR 2884.00
  • EUR 811.00
  • EUR 396.00
  • EUR 202.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Lys152~Glu381)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with PE.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human, Mouse)

4-PAL650Mu01
  • EUR 266.00
  • EUR 2813.00
  • EUR 694.00
  • EUR 337.00
  • EUR 222.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Leu156~Glu381)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1)

qPCR Control Template Kit

G929 100 rxns
EUR 148

Green qPCR SuperMix UDG

20-abx098032
  • EUR 1135.00
  • EUR 453.00
  • EUR 662.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

Top Green qPCR SuperMix

20-abx098033
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.

Tip Green qPCR SuperMix

20-abx098034
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.

Library Quantification qPCR SuperMix

20-abx098896
  • EUR 230.00
  • EUR 411.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

Eva QPCR SuperMix Kit

K5052200 200 reactions
EUR 211
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Eva QPCR SuperMix Kit

K5052400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit

K5053200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit

K5053400 400 reactions
EUR 299
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QPCR Positive Control Kit

M1126-100
EUR 403

Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 999
  • Category: Lineage Profilers

Regulatory RNA qPCR Profiler

RA950A-1 20 rxn
EUR 880
  • Category: Long Non-coding RNA Tools

Retrovirus qPCR Quantification Kit

K1472-100 100 Rxns Ask for price

Lentivirus qPCR Quantification Kit

K1471-100 100 Rxns
EUR 298

Amino Particle Array Kit

APAK-3567-7K 7X1 mL
EUR 558
Description: Amino Particle Array KitGentaur offers a wide variety of uniform particles, ranging in size from 0.05 um to 20 um, with surface functional groups such as carboxyl or amino groups for covalent coupling of proteins or other ligands.

Fluorescent Particle Array Kit

FPAK-4058--10K 10 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Blue Particle Array Kit

PAK-3567-7K 7X1 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Blue Particle Array Kit

PAK-4067-8K 8X1 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Blue Particle Array Kit

PAK-5067-10K 10X1 mL
EUR 939
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Blue Particle Array Kit

PAK-5067-5A 5X1 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Blue Particle Array Kit

PAK-5067-5B 5X1 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Blue Particle Array Kit

PAK-7067-9K 9X1 mL
EUR 939
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

FDA Standard Tissue Array

T8234701-1 1 slides
EUR 214
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

FDA Standard Tissue Array

T8234701-5 5 slides
EUR 654
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

CA125 antibody array kit

VArk-Lsx008 1 mg
EUR 664
Description: CA125 can be used as a tumor marker to identify the type of tumor and check if the treatment is working.

Ferritin antibody array kit

VArk-Lsx009 1 mg
EUR 419
Description: Used for detection of Ferritin

Microalbuminuria antibody array kit

VArk-Lsx010 1 mg
EUR 419
Description: Used to detect early signs of kidney damage

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human), APC-Cy7

4-PAL650Hu01-APC-Cy7
  • EUR 614.00
  • EUR 7042.00
  • EUR 1858.00
  • EUR 821.00
  • EUR 337.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Lys152~Glu381)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with APC-Cy7.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human, Mouse), APC

4-PAL650Mu01-APC
  • EUR 374.00
  • EUR 3689.00
  • EUR 1016.00
  • EUR 481.00
  • EUR 232.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Leu156~Glu381)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with APC.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human, Mouse), Biotinylated

4-PAL650Mu01-Biotin
  • EUR 332.00
  • EUR 2763.00
  • EUR 803.00
  • EUR 411.00
  • EUR 228.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Leu156~Glu381)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with Biotin.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human, Mouse), Cy3

4-PAL650Mu01-Cy3
  • EUR 457.00
  • EUR 4877.00
  • EUR 1313.00
  • EUR 600.00
  • EUR 267.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Leu156~Glu381)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with Cy3.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human, Mouse), FITC

4-PAL650Mu01-FITC
  • EUR 319.00
  • EUR 2971.00
  • EUR 832.00
  • EUR 405.00
  • EUR 205.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Leu156~Glu381)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with FITC.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human, Mouse), HRP

4-PAL650Mu01-HRP
  • EUR 341.00
  • EUR 3213.00
  • EUR 897.00
  • EUR 433.00
  • EUR 217.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Leu156~Glu381)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with HRP.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human, Mouse), PE

4-PAL650Mu01-PE
  • EUR 319.00
  • EUR 2971.00
  • EUR 832.00
  • EUR 405.00
  • EUR 205.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Leu156~Glu381)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with PE.

ViraDuctin Adenovirus Transduction Kit (10 Transductions)

AD-200 10 transductions
EUR 415
Description: Adenoviral infection of most cells is mediated largely by the coxsackievirus-adenovirus receptor (CAR). In many cells, however, this receptor is either absent or present at extremely low levels, reducing the efficiency of adenovirus uptake. ViraDuctin Adenovirus Transduction Reagent is designed specifically to increase the efficiency of adenoviral transduction without regard to the level of CAR expression on the surface of the target cells.

ViraDuctin Adenovirus Transduction Kit (50 Transductions)

AD-201 50 transductions
EUR 949
Description: Adenoviral infection of most cells is mediated largely by the coxsackievirus-adenovirus receptor (CAR). In many cells, however, this receptor is either absent or present at extremely low levels, reducing the efficiency of adenovirus uptake. ViraDuctin Adenovirus Transduction Reagent is designed specifically to increase the efficiency of adenoviral transduction without regard to the level of CAR expression on the surface of the target cells.

ViraDuctin AAV Transduction Kit (10 Transductions)

AAV-200 10 transductions
EUR 421
Description: The process by which adeno-associated virus (AAV) infects host cells includes viral binding and entry, intracellular trafficking, nuclear transport, and viral second strand DNA synthesis. The second strand DNA synthesis has been shown to be the rate limiting step, which leads to inefficient transduction by AAV vectors. ViraDuctin AAV Transduction Kit is a proprietary, multi-reagent formulation designed to increase the efficiency of transduction by AAV vectors. Reagent preparation takes only 10 minutes prior to host cell infection.

ViraDuctin AAV Transduction Kit (50 Transductions)

AAV-201 50 transductions
EUR 972
Description: The process by which adeno-associated virus (AAV) infects host cells includes viral binding and entry, intracellular trafficking, nuclear transport, and viral second strand DNA synthesis. The second strand DNA synthesis has been shown to be the rate limiting step, which leads to inefficient transduction by AAV vectors. ViraDuctin AAV Transduction Kit is a proprietary, multi-reagent formulation designed to increase the efficiency of transduction by AAV vectors. Reagent preparation takes only 10 minutes prior to host cell infection.

ViraDuctin Lentivirus Transduction Kit (40 Transductions)

LTV-200 40 transductions
EUR 345
Description: Lentiviral expression has many advantages over other viruses, including the ability to infect both proliferating and non-proliferating cells. The efficiency of lentivirus infection of target cells, however, tends to be lower. Additives such as Polybrene can increase transduction efficiencies, but even then only a small fraction of lentiviral vectors can trasduce many target cell lines. Our ViraDuctin Lentivirus Transduction Kit provides superior lentiviral transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene. This system is ideal for many primary cells as well as immobilized cells.

ViraDuctin Lentivirus Transduction Kit (200 Transductions)

LTV-201 200 transductions
EUR 751
Description: Lentiviral expression has many advantages over other viruses, including the ability to infect both proliferating and non-proliferating cells. The efficiency of lentivirus infection of target cells, however, tends to be lower. Additives such as Polybrene can increase transduction efficiencies, but even then only a small fraction of lentiviral vectors can trasduce many target cell lines. Our ViraDuctin Lentivirus Transduction Kit provides superior lentiviral transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene. This system is ideal for many primary cells as well as immobilized cells.

ViraDuctin Retrovirus Transduction Kit (40 Transductions)

RV-200 40 transductions
EUR 363
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

ViraDuctin Retrovirus Transduction Kit (200 Transductions)

RV-201 200 transductions
EUR 780
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Polyclonal Antibody (Human, Mouse), APC-Cy7

4-PAL650Mu01-APC-Cy7
  • EUR 628.00
  • EUR 7258.00
  • EUR 1912.00
  • EUR 842.00
  • EUR 344.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: KHDRBS1 (Leu156~Glu381)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1). This antibody is labeled with APC-Cy7.

Pig Signal Recognition Particle 9kDa ELISA kit

E07S0185-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Signal Recognition Particle 9kDa in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Signal Recognition Particle 9kDa ELISA kit

E07S0185-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Signal Recognition Particle 9kDa in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Signal Recognition Particle 9kDa ELISA kit

E07S0185-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Signal Recognition Particle 9kDa in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Signal Recognition Particle Antibody ELISA kit

E07S0285-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Signal Recognition Particle Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Signal Recognition Particle Antibody ELISA kit

E07S0285-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Signal Recognition Particle Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Signal Recognition Particle Antibody ELISA kit

E07S0285-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Signal Recognition Particle Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Extracellular Signal Regulated Kinase ELISA kit

E07E0171-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Extracellular Signal Regulated Kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Extracellular Signal Regulated Kinase ELISA kit

E07E0171-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Extracellular Signal Regulated Kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Extracellular Signal Regulated Kinase ELISA kit

E07E0171-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Extracellular Signal Regulated Kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Paraffin Tissue Section Array - Human Adult Normal Tissue, Array I (64 Specimens)

T8234708-2 2 slides
EUR 307
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Array - Human Adult Normal Tissue, Array I (64 Specimens)

T8234708-5 5 slides
EUR 579
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

HotTaq Probe qPCR Mix (ROX)

BT11001 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (Capillary)

BT11003 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Universal Mix

BT11004 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)

BT11101 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (Capillary)

BT11103 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Universal Mix

BT11104 250rxn
EUR 138
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

SYBR Green qPCR Master Mix

HY-K0501 5 mL (500 rxns)
EUR 263

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 291

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 690.00
  • EUR 565.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Power Eva QPCR SuperMix Kit

K5057200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Power Eva QPCR SuperMix Kit

K5057400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

BrightGreen 5X qPCR MasterMix-iCycler

MasterMix-5C 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-ROX

MasterMix-5P 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-iCycler

MasterMix-5PC 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-Multiplex

MasterMix-5PM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 5X qPCR MasterMix-ROX

MasterMix-5R 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

KiloGreen 2X qPCR MasterMix-iCycler

MasterMix-KC 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-ROX

MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-iCycler

MasterMix-mC 4 x 1.25 ml for 500 reactions
EUR 151

BrightGreen miRNA qPCR MasterMix-ROX

MasterMix-mR 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-ROX

MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-iCycler

MasterMix-PC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-Multiplex

MasterMix-PM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

Methylamp MS-qPCR Fast Kit

P-1028 200 Reactions
EUR 496.6
Description: fast delivery possible

AceQ qPCR Probe Master Mix

Q112-02 500 rxn (20 μl/rxn)
EUR 221

This in the end impacts the yield, dietary worth, and high quality of fruit. Particularly, the principle motive of the postharvest storage loss brought on by physiological problems is the improper proportion of mineral nutrient components. Lately, many vital mineral transport proteins and their regulatory parts are more and more revealed, which make drastic progress in understanding the molecular mechanisms for mineral vitamin (N, P, Okay, Ca, and Fe) in varied points together with plant development, fruit growth, high quality, vitamin, and postharvest storage.

Methane-rich water induces bulblet formation of scale cuttings in Lilium davidii var. unicolor by regulating the signal transduction of phytohormones and their levels

Methane-rich water induces bulblet formation of scale cuttings in Lilium davidii var. unicolor by regulating the signal transduction of phytohormones and their levels

Earlier research have proven that methane (CH4 ) has selling roles within the adventitious root (AR) and lateral root (LR) formation in crops. Nevertheless, whether or not CH4 may set off the bulblet formation in scale reducing of Lilium davidii var. unicolor has not been elucidated. To achieve perception into the impact of CH4 on the bulblet formation, completely different concentrations (1, 10,50 and 100%) of methane-rich water (MRW) and distilled water have been utilized to deal with the size cuttings of Lilium. We noticed that therapy with 100% MRW clearly induced the bulblet formation in scale cuttings.

To discover the mechanism of CH4 -induced bulblet formation, the transcriptome of scales was analyzed. A complete of 2078 differentially expressed genes (DEGs) have been recognized. The DEGs have been labeled into completely different metabolic pathways, particularly phenylpropanoid biosynthesis, starch and sucrose metabolism and plant sign transduction. Of those, roughly 38 candidate DEGs concerned within the plant sign transduction have been additional studied. As well as, the expression of AP2-ERF/ERF, WRKY, GRAS, ARF and NAC transcription components have been modified by MRW therapy, suggesting their potential involvement in bulblet formation.

As for hormones, exogenous IAA, GA and ABA may induce the bulblet formation. Further experiments advised that MRW may enhance the endogenous IAA, GA, and JA ranges, however lower the degrees of ABA throughout bulblet formation, which confirmed that increased IAA, GA, JA ranges and decrease ABA content material may facilitate bulblet formation. As well as, the degrees of endogenous hormones have been according to the expression stage of genes concerned in phytohormone sign transduction. Total, this research has revealed that CH4 may enhance the bulblet formation of reducing scales in Lilium by regulating the expression of genes associated to phytohormone sign transduction and transcription components, in addition to by altering the endogenous hormone ranges.

An effector-reporter system to review mobile sign transduction in strawberry fruit (Fragaria ananassa)

An effector-reporter system is a robust device used to review mobile sign transduction, however this system has been historically utilized in protoplasts. The same system to review mobile sign transduction in fruits has not but been established. On this research, we aimed to ascertain an effector-reporter system for strawberry fruit, a mannequin nonclimacteric fruit. We first investigated the traits of transient gene expression in strawberry fruits and located marked variation in gene expression ranges amongst particular person fruits, and this variation has sophisticated the institution of a technical system.

To beat this problem, we investigated a sampling technique based mostly on a statistical evaluation of the exercise sample of 4 completely different reporters (GUS, GFP, FLuc, and RLuc) amongst particular person fruits and combos of pairs of reporters (GUS/GFP and RLuc/FLuc). Based mostly on an optimized sampling technique, we lastly established a step-by step protocol for the effector/reporter assay. Utilizing FaMYB10 and FaWRKY71 because the effectors and GUS pushed by the FaCHS promoter because the reporter, we demonstrated that this effector/reporter system was sensible and dependable. This effector/reporter approach will contribute to an in-depth exploration of the signaling mechanism for the regulation of strawberry fruit ripening.

The first cilium tasks from the floor of most vertebrate cells, the place it senses extracellular alerts to manage various mobile processes throughout tissue growth and homeostasis. Dysfunction of major cilia underlies the pathogenesis of extreme ailments, generally known as ciliopathies. Major cilia comprise a novel protein repertoire that’s distinct from the cell physique and the plasma membrane, enabling the spatially managed transduction of extracellular cues. G-protein coupled receptors (GPCRs) are key in sensing environmental stimuli which can be transmitted by way of second messenger signaling right into a mobile response. Right here, we are going to give an summary of the function of GPCR signaling in major cilia, and the way ciliary GPCR signaling might be focused by pharmacology, chemogenetics, and optogenetics.

Methane-rich water induces bulblet formation of scale cuttings in Lilium davidii var. unicolor by regulating the signal transduction of phytohormones and their levels

The function and mechanism of β-arrestin2 in sign transduction

β-arrestin2 is a ubiquitously expressed scaffold protein localized on the cytoplasm and plasma membrane. Additional investigations have revealed that β-arrestin2 not solely mediates the desensitization of GPCRs but in addition serves as a multifunctional scaffold to mediate receptor internalization, kinase activation, and regulation of assorted signaling pathways, reminiscent of TLR4/NF-κB, MAPK, Wnt, TGF-β, and AMPK/mTOR pathways. β-arrestin2 regulates cell invasion, migration, autophagy, angiogenesis, and anti inflammatory results by regulating varied signaling pathways, which play an important function in lots of physiological and pathological processes.

This paper critiques the construction and performance of β-arrestin2, the regulation of β-arrestin2 based mostly signaling pathways. The function and mechanism of β-arrestin2 signaling have been delineated in adequate element. The prospect of regulating the expression and exercise of β-arrestin2 in multisystem ailments holds substantial therapeutic promise. Important progress has been made prior to now 20 years in the direction of the understanding of the essential mechanisms underlying most cancers development and angiogenesis. On this context, receptor tyrosine kinases (RTKs) play a pivotal function in cell proliferation, differentiation, development, motility, invasion, and angiogenesis, all of which contribute to tumor development and development.

Mutations in RTKs result in irregular sign transductions in a number of pathways reminiscent of Ras-Raf, MEK-MAPK, PI3K-AKT and mTOR pathways, which impacts protein translation and a variety of organic features together with cell proliferation, survival, migration and vascular permeability. It was initially discovered to bind to GPCRs, uncoupling G proteins and receptors’ binding and inhibiting the sign transduction of the GPCRs.

Defective T cell receptor-mediated signal transduction in memory CD4 T lymphocytes exposed to superantigen or anti-T cell receptor antibodies.

Defective T cell receptor-mediated signal transduction in memory CD4 T lymphocytes exposed to superantigen or anti-T cell receptor antibodies.

Lymphocytes should promote protecting immune responses whereas nonetheless sustaining self-tolerance.

Stimulation by way of the T cell receptor (TCR) can result in distinct responses in naive and reminiscence CD4 T cells. Whereas peptide antigen stimulates each naive and reminiscence T cells, soluble anti-CD3 antibodies and bacterial superantigens stimulate solely naive T cells to proliferate and secrete cytokines.

Additional, superantigens, like staphylococcal enterotoxin B (SEB), trigger reminiscence T cells to grow to be anergic whereas soluble anti-CD3 doesn’t. Within the current report, we present that sign transduction by way of the TCR is impaired in reminiscence cells uncovered to both anti-CD3 or SEB.

A block in signaling results in impaired activation of the kinase ZAP-70 in order that downstream indicators and cell proliferation don’t happen. We additional present that the signaling defect is exclusive to every agent.

In anti-CD3-treated reminiscence T cells, the src kinase Lck is simply transiently activated and doesn’t phosphorylate and activate ZAP-70. In SEB-treated reminiscence T cells, ZAP-70 doesn’t work together with the TCR/CD3 complicated to grow to be accessible to Lck. Lastly, we offer proof that various signaling pathways are initiated in SEB-treated reminiscence cells.

Altered signaling, indicated by an elevation in exercise of the src kinase Fyn, could also be accountable for reminiscence cell anergy brought on by SEB. Thus, differentiation of naive T cells into reminiscence cells is accompanied by alterations in TCR-mediated signaling that may promote heightened recall immunity or particular tolerance.

Novel human monoclonal antibodies to insulin-like progress issue (IGF)-II that potently inhibit the IGF receptor kind I sign transduction operate.

The insulin-like progress issue (IGF) system performs an necessary position in quite a lot of physiologic processes and in ailments similar to most cancers.

Though the position of the IGF system in most cancers has been acknowledged a few years in the past, elements of the system have solely not too long ago been focused and proven to have an effect on cell transformation, proliferation, survival, motility, and migration in tissue cultures and in mouse fashions of most cancers.

Now we have been hypothesizing that concentrating on IGF-II along with blocking its interplay with the IGF receptor kind I (IGF-IR) would additionally permit to dam that portion of the sign transduction by way of the insulin receptor that is because of its interplay with IGF-II. Reducing its stage may additionally not induce up-regulation of its manufacturing as for IGF-I.

Lastly, concentrating on a diffusable ligand as IGF-II might not require penetration of the antibody inside tumors however might shift the equilibrium to IGF-II complexed with antibody so the ligand focus would lower within the tumor setting with out the necessity for the antibody to penetrate the tumor.

Right here, we describe the identification and characterization of three novel anti-IGF-II absolutely human monoclonal antibodies. They certain with excessive (subnanomolar) affinity to IGF-II, didn’t cross-react with IGF-I and insulin, and potently inhibited sign transduction mediated by the IGF-IR interplay with IGF-II.

Essentially the most potent neutralizer, IgG1 m610, inhibited phosphorylation of the IGF-IR and the insulin receptor, in addition to phosphorylation of the downstream kinases Akt and mitogen-activated protein kinase with an IC(50) of the order of 1 nmol/L at IGF-II focus of 10 nmol/L.

It additionally inhibited progress of the prostate most cancers cell line DU145 and migration of the breast most cancers line cells MCF-7. These outcomes point out an immunotherapeutic potential of IgG1 m610 seemingly together with different antibodies and anticancer medication however solely additional experiments in mouse fashions of most cancers and human scientific trials might consider this chance.