B-cell-intrinsic function of TAPP adaptors in controlling germinal center responses and autoantibody production in mice.

Control of B-cell signaltransduction is critical to prevent production of pathological autoantibodies. Tandem PH domain containing proteins (TAPPs) specifically bind PI(3,4)P2, a phosphoinositide product generated by PI 3-kinases and the phosphatase SHIP. TAPP KI mice bearing PH domain-inactivating mutations in both TAPP1 and TAPP2 genes, uncoupling them from PI(3,4)P2, exhibit increased BCR-induced activation of the kinase Akt and develop lupus-like characteristics including anti-DNA antibodies and deposition of immune complexes in kidneys.

Here, we find that TAPP KI mice develop chronic germinal centers (GCs) with age and show abnormal expression of B-cell activation and memory markers. Upon immunization with T-dependent Ag, TAPP KI mice develop functional but abnormally large GCs, associated with increased GC B-cell survival. Disruption of chronic GCs in TAPP KI mice by deletion of the costimulatory molecule ICOS abrogate anti-DNA and anti-nuclear antibody production in TAPP KI mice, indicating an essential role for GCs.

CKMM protein

30-1084U 100 units
EUR 279
Description: Highly pure native Human CKMM protein

CKMM protein

30-1369 500 ug
EUR 392
Description: Recombinant CKMM protein

CKMM protein

30-AC60 1 mg
EUR 398
Description: Purified native Human CKMM protein

CKMM protein

30-AC61 100 ug
EUR 271
Description: Purified recombinant CKMM protein

CKMM antibody

20C-CR3014G 1 ml
EUR 225
Description: Goat polyclonal CKMM antibody

CKMM antibody

70R-1984 50 ug
EUR 467
Description: Rabbit polyclonal CKMM antibody raised against the N terminal of CKM

CKMM antibody

70R-1985 50 ug
EUR 467
Description: Rabbit polyclonal CKMM antibody raised against the middle region of CKM

CKMM antibody

70-XG47 1 mg
EUR 324
Description: Affinity purified Goat polyclonal CKMM antibody

CKMM antibody

70R-15270 100 ug
EUR 327
Description: Rabbit polyclonal CKMM antibody

CKMM antibody

10C-CR3014M1 1 mg
EUR 495
Description: Mouse monoclonal CKMM antibody

CKMM antibody

10C-CR3014M2 1 mg
EUR 379
Description: Mouse monoclonal CKMM antibody

CKMM antibody

10C-CR3014M3 1 mg
EUR 495
Description: Mouse monoclonal CKMM antibody

CKMM antibody

10-C41A 1 mg
EUR 495
Description: Mouse monoclonal CKMM antibody

CKMM antibody

10-C41B 1 mg
EUR 379
Description: Mouse monoclonal CKMM antibody

CKMM antibody

20-CG75 1 ml
EUR 173
Description: Goat polyclonal CKMM antibody

CKMM antibody

20-CR75 1 ml
EUR 173
Description: Rabbit polyclonal CKMM antibody

CKMM antibody

10R-8516 100 ul
EUR 393
Description: Mouse monoclonal CKMM antibody

CKMM antibody

70R-33149 100 ug
EUR 435
Description: Rabbit polyclonal CKMM antibody

CKMM, Human

LF-P0096 0.2 mg
EUR 303
Description: CKMM, Human protein

CKMM Blocking Peptide

33R-3630 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CKM antibody, catalog no. 70R-1985

CKMM Blocking Peptide

33R-9549 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CKM antibody, catalog no. 70R-1984

Mouse CKMM Antibody

32938-05111 150 ug
EUR 261

Rat CKMM Antibody

32939-05111 150 ug
EUR 261

CKMM antibody (HRP)

60R-1648 100 ug
EUR 327
Description: Rabbit polyclonal CKMM antibody (HRP)

CKMM antibody (FITC)

60R-1649 100 ug
EUR 327
Description: Rabbit polyclonal CKMM antibody (FITC)

CKMM antibody (biotin)

60R-1650 100 ug
EUR 349
Description: Rabbit polyclonal CKMM antibody (biotin)

anti-CKMM (3C3)

LF-MA0399 100 ul
EUR 334
Description: Mouse monoclonal to CKMM

anti-CKMM (11B11)

LF-MA0400 100 ul
EUR 334
Description: Mouse monoclonal to CKMM

CKMM Type 1 protein

30R-AC026 1 mg
EUR 791
Description: Purified recombinant CKMM Type 1 protein

Mouse CKMM Antibody (Biotin Conjugate)

32938-05121 150 ug
EUR 369

Rat CKMM Antibody (Biotin Conjugate)

32939-05121 150 ug
EUR 369

Human ckmm PicoKine ELISA Kit

EK1751 96 wells
EUR 425
Description: For quantitative detection of human ckmm in cell culture supernates, serum and plasma (heparin, EDTA).

Mouse CKMM AssayLite Antibody (FITC Conjugate)

32938-05141 150 ug
EUR 428

Mouse CKMM AssayLite Antibody (RPE Conjugate)

32938-05151 150 ug
EUR 428

Mouse CKMM AssayLite Antibody (APC Conjugate)

32938-05161 150 ug
EUR 428

Mouse CKMM AssayLite Antibody (PerCP Conjugate)

32938-05171 150 ug
EUR 471

Rat CKMM AssayLite Antibody (FITC Conjugate)

32939-05141 150 ug
EUR 428

Rat CKMM AssayLite Antibody (RPE Coinjugate)

32939-05151 150 ug
EUR 428

Rat CKMM AssayLite Antibody (APC Conjugate)

32939-05161 150 ug
EUR 428

Rat CKMM AssayLite Antibody (PerCP Conjugate)

32939-05171 150 ug
EUR 471

Human Creatine Kinase M-type (CKMM) Antibody

11281-05011 150 ug
EUR 217

Recombinant Human Creatine Kinase, Muscle/CKMM (C-6His)

CC96-10ug 10ug
EUR 141
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,10%Glycerol,pH7.5.

Recombinant Human Creatine Kinase, Muscle/CKMM (C-6His)

CC96-1mg 1mg
EUR 1674
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,10%Glycerol,pH7.5.

Recombinant Human Creatine Kinase, Muscle/CKMM (C-6His)

CC96-500ug 500ug
EUR 1186
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,10%Glycerol,pH7.5.

Recombinant Human Creatine Kinase, Muscle/CKMM (C-6His)

CC96-50ug 50ug
EUR 263
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,10%Glycerol,pH7.5.

2-Propyl-β-D-glucuronide, 2 mg

0904-2 2 mg
EUR 162

IL-2 Interleukin-2 Human Recombinant Protein, His Tag

PROTP60568-2 Regular: 10ug
EUR 317
Description: Interleukin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 133 amino acids fragment (21-153) having a molecular weight of 20kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. _x000D_ The IL-2 His is purified by proprietary chromatographic techniques._x000D_

Human Creatine Kinase M-type (CKMM) Antibody (Biotin Conjugate)

11281-05021 150 ug
EUR 276

BMP-2 Bone Morphogenetic Protein-2 Human Recombinant Protein, Monomer

PROTP12643-2 Regular: 20ug
EUR 317
Description: Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 115 amino acids (283-396) and having a molecular mass of 13009 Dalton. ;The BMP-2 is purified by proprietary chromatographic techniques.

Individual Reaction Mix 2

G065-2 200 reactions
EUR 167

Anti-Fascin 2/FSCN2 Antibody

A07840-2 100ug/vial
EUR 294

Anti-EIF4A1/2/3 Antibody

A03922-2 100ug/vial
EUR 334

Anti-ErbB 2/ERBB2 Antibody

A00010-2 100ug/vial
EUR 334

Anti-Bcl-2/BCL2 Antibody

A00040-2 100ug/vial
EUR 334

Anti-Angiopoietin-2/ANGPT2 Antibody

A00370-2 100ug/vial
EUR 334

Dr. P Kit-Solution 2

K2021010-2 6 ml
EUR 120
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Recombinant Human TFF-2 Protein

PROTQ03403-2 20ug
EUR 317
Description: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data suggests a potential role for TFF2 in acute and chronic asthma (Nikolaidis, N.M. et al. Am. Journal Respir. Cell Mol. Biol. (2003) 4: 458-464). Recombinant human TFF2 is a 12.0 kDa polypeptide of 107 amino acid residues, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Recombinant Human BD-2 Protein

PROTO15263-2 20ug
EUR 317
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-2 is a 4.3 kDa protein containing 41 amino acid residues.

Recombinant Human Relaxin-2 Protein

PROTP04090-2 25ug
EUR 317
Description: Relaxin-2 is a peptide hormone structurally related to insulin, which is expressed in the placenta, decidua, prostate, and in the ovary during pregnancy. Of the three known relaxin genes, Relaxin-2 is the only relaxin known to circulate in the blood. Relaxin-2 binds specifically to the LGR7 and LGR8 receptors, previously identified as an “orphan” G protein coupled receptors. Signaling by Relaxin-2 through its target receptors enhances the growth of pubic ligaments and ripening of the cervix during birth. Recombinant Relaxin-2 is a nonglycosylated 6.0 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 29 amino acid B-chain.

Recombinant Murine IL-2 Protein

PROTP04351-2 20ug
EUR 317
Description: IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant murine IL-2 is a 17.2 kDa protein, containing 149 amino acid residues.

Recombinant Human PAI-2 Protein

PROTP05120-2 10ug
EUR 317
Description: PAI-2 is an inhibitory serpin expressed mainly in keratinocytes, activated monocytes, and placental trophoblasts. It exists predominantly as a 47 kDa nonglycosylated intracellular protein which can be induced to be secreted as 60 kDa glycoprotein. The glycosylated and unglycosylated forms of PAI-2 are equally effective as inhibitors of urokinase-type plasminogen activator (uPA), the only established physiological target of this serpin. PAI-2 has a unique ability to form dormant polymers spontaneously and reversibly under physiological conditions. The physiological relevance of this property, which is neither a consequence of any mutation in the PAI-2 gene nor associated with any known disorder, is still unclear. However, it appears that the formation of intracellular dormant polymers may be important for the controlled release of the inhibitor from PAI-2 producing cells. Plasma levels of PAI-2 are usually low or undetectable, except during pregnancy and in some forms of monocytic leukemia. Secretion of PAI-2 from the placenta normally occurs during the third trimester of pregnancy and accounts for the dramatic increase in PAI-2 levels (up to 250 ng/ml), which are maintained at these levels until postpartum, and then rapidly decline. In addition to its vital role in protecting the placenta from degradation by uPA and/or uPA-activated proteases, PAI-2 has been shown to be essential for the prevention of metastatic spread of neck, lung and breast cancers. The beneficial effect of PAI-2 seen in these studies is presumed to stem from its ability to inhibit uPA-dependent cell dissemination. PAI-2 has also been reported to inhibit keratinocyte proliferation, and to participate in the innate immune response during viral infection. Recombinant human PAI-2 is a 415-residue nonglycosylated protein.

Recombinant Human MMP-2 Protein

PROTP08253-2 10ug
EUR 317
Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-2 is a secreted collagenase with specificity toward Type IV, V, VII, and X collagens. Recombinant human MMP-2 is a 62.0 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (552 amino acids).

pLenti-CARM1 shRNA-2 Plasmid

PVTBAV03691-2 2 ug
EUR 356

pLenti-CTLA4 shRNA-2 Plasmid

PVTBAV05689-2 2 ug
EUR 356

pLenti-FOXM1 shRNA-2 Plasmid

PVTBAV08732-2 2 ug
EUR 356

pLenti-JUN shRNA-2 Plasmid

PVTBAV11741-2 2 ug
EUR 356

pLenti-LHX6 shRNA-2 Plasmid

PVTBAV12881-2 2 ug
EUR 356

pLenti-MAGEA3 shRNA-2 Plasmid

PVTBAV13661-2 2 ug
EUR 356

pLenti-RUNX3 shRNA-2 Plasmid

PVTBAV20583-2 2 ug
EUR 356

pLenti-Slc7a11 shRNA-2 Plasmid

PVTBAV21973-2 2 ug
EUR 356

pLenti-STAT3 shRNA-2 Plasmid

PVTBAV22921-2 2 ug
EUR 356

pLenti-XRCC5 shRNA-2 Plasmid

PVTBAV26238-2 2 ug
EUR 356

Mouse FGF-2 Recombinant Protein

R00121-2 5ug/vial
EUR 259
Description: FGF basic (FGF2) is a multipotential fibroblast growth factor that stimulates and supports proliferation, migration and differentiation. Mouse FGF basic (FGF-2) Recombinant Protein is purified FGF basic (FGF-2) produced in yeast.

Chicken IL-2 Recombinant Protein

R00387-2 5ug/vial
EUR 259
Description: Interleukin-2 (IL-2) is a cytokine produced by T-helper cells in response to antigenic or mitogenic stimulation. It is required for T-cell proliferation and other activities crucial to the regulation of the immune response. Chicken IL-2 Recombinant Protein is purified interleukin-2 produced in yeast.

Anti-p56Dok-2 (Ab-299) Antibody

A07956-2 100ul
EUR 397
Description: Rabbit Polyclonal p56Dok-2 (Ab-299) Antibody. Validated in IF, IHC, WB and tested in Human.

Ethyl-β-D-glucuronide, 2 mg

0901-2 2 mg
EUR 162

Ethyl sulfate, sodium salt, 2 mg

0902-2 2 mg
EUR 69

Propyl-β-D-glucuronide, 2 mg

0903-2 2 mg
EUR 162

Anti-VEGF Receptor 2/KDR Antibody

A00901-2 100ug/vial
EUR 334

Anti-Adiponectin Receptor 2 (mouse) Antibody

A02218-2 200ug
EUR 498
Description: Goat Polyclonal Adiponectin Receptor 2 (mouse) Antibody. Validated in IF, IHC and tested in Mouse.

Anti-Anterior Gradient 2/AGR2 Antibody

A02922-2 100ug/vial
EUR 334

Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody

A00084-2 100ug/vial
EUR 294

Anti-beta 2 Microglobulin/B2m Antibody

A00456-2 100ug/vial
EUR 294

Anti-HIF-2-alpha/EPAS1 Antibody

PA1129-2 100ug/vial
EUR 294

Anti-Bcl-2 Rabbit Monoclonal Antibody

M00040-2 100ug/vial
EUR 397
Description: Rabbit Monoclonal Bcl-2 Antibody. Validated in IF, WB and tested in Human, Mouse.

ErbB2 ErbB-2 Human Recombinant Protein

PROTP04626-2 Regular: 20ug
EUR 317
Description: ErbB-2 Human Recombinant is a 43.4 kDa protein containing 397 amino acid residues of the human Herstatin, and an extra Methionine at N-Terminal (underlined), produced in E.coli.

Human Creatine Kinase M-type (CKMM) AssayLite Antibody (FITC Conjugate)

11281-05041 150 ug
EUR 428

Human Creatine Kinase M-type (CKMM) AssayLite Antibody (RPE Conjugate)

11281-05051 150 ug
EUR 428

Human Creatine Kinase M-type (CKMM) AssayLite Antibody (APC Conjugate)

11281-05061 150 ug
EUR 428

Human Creatine Kinase M-type (CKMM) AssayLite Antibody (PerCP Conjugate)

11281-05071 150 ug
EUR 471

Mouse Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-82102 1 Kit Ask for price

Rat Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-82597 1 Kit Ask for price

Human Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-96123 1 Kit Ask for price

Ethyl-β-D-glucuronide-d5, 2 mg

0901D-2 2 mg
EUR 386

Ethyl sulfate-d5, sodium salt, 2 mg

0902D-2 2 mg
EUR 92

Ethyl-β-D-glucuronide-13C6, 2 mg

0905-2 2 mg
EUR 366

Anti-Beta 2 Microglobulin Antibody (monoclonal, 2H10)

M00456-2 100ug/vial
EUR 334

ENO2 Neurone Specific Enolase 2 Human protein

PROTP09104-2 Regular: 20ug
EUR 317
Description: Human Neurone Specific Enolase produced in Human CNS having a molecular mass of 45kDa.

Moreover, TAPP KI B cells are sufficient to drive chronic GC responses and recapitulate the autoimmune phenotype in BM chimeric mice. Our findings demonstrate a B-cell-intrinsic role of TAPP-PI(3,4)P2 interaction in regulating GC responses and autoantibody production and suggest that uncontrolled Akt activity in B cells can drive autoimmunity.