General description
FastStart™ Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and across multiple instrument platforms. This modified thermostable recombinant Taq DNA polymerase is inactive at temperatures below +75°C but is activated by a 2-4 minute heat activation step at +95°C. Because it is inactive at low temperatures, FastStart™ Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that can form at these temperatures. FastStart™ Taq DNA Polymerase is an ideal tool for hot-start PCR because the enzyme remains inactive during PCR setup and before the initial denaturation step.
Request
FastStart™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA polymerase. The enzyme is inactive at +15 to +25°C during PCR setup and then activated at +95°C during initial denaturation. This enzyme offers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot-start PCR because the enzyme remains inactive during the PCR setup and before the initial denaturation step.
It can be requested for:
- PCR
- multiplex PCR
- Difficult templates, eg secondary structures or GC-rich sequences
- Automated PCR, e.g. e.g., handling at room temperature
- Hot Start PCR up to 3kb
- Hot start RT-PCR up to 3kb
- Quantitative reverse transcription PCR (RT-qPCR)
- bisulfite-specific PCR
Use FastStart™ Taq DNA Polymerase, DNA pack with a ready-to-use PCR nucleotide mix.
Features and Benefits
FastStart Taq DNA Polymerase is a modified recombinant Taq DNA Polymerase, inactive below +75°C. The kit includes an optimized PCR buffer and GC-RICH solution to handle a wide range of templates. High enzyme stability allows pipetting using robotic stations.
- Greater specificity, sensitivity and performance:
Hot start PCR makes it easy to set up the PCR.
- Use the robotic setup.
Use this stable enzyme mix for 24 hours at +15 to +25°C.
- Avoid PCR carryover contamination.
Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes
Packaging
1 kit containing 5 components
Quality
Each lot is functionally tested using human genomic DNA and primers specific for the 365 bp fragment of the human tPA gene and a 284 bp fragment of the Apo E gene with 74% GC content. Each lot is also tested for the absence of exonucleases and endonucleases and for shear activity.
Unit Definition
1 μg of M13mp9ss DNA, 0.3 μg of the M13 sequencing primer and 0.1 μCi [α-32P] dCTP are incubated with varying amounts of FastStart Taq DNA polymerase units in 50 μl of incubation buffer at +65 °C. for 60 min. The amount of incorporated dNTPs is determined by precipitation with trichloroacetic acid.
Unit assay: 1 μg of M13mp9ss DNA, 0.3 μg of the M13 sequencing primer, and 0.1 μCi [α-32P]dCTP are incubated with varying amounts of units of FastStart Taq DNA polymerase in 50 μL of incubation buffer at +65°C for 60 min. The amount of incorporated dNTPs is determined by precipitation with trichloroacetic acid.
Volume Activity: 5 U/μl
Other notes
For life science research only. It should not be used in diagnostic procedures.