B-cell-intrinsic function of TAPP adaptors in controlling germinal center responses and autoantibody production in mice.

Control of B-cell signaltransduction is critical to prevent production of pathological autoantibodies. Tandem PH domain containing proteins (TAPPs) specifically bind PI(3,4)P2, a phosphoinositide product generated by PI 3-kinases and the phosphatase SHIP. TAPP KI mice bearing PH domain-inactivating mutations in both TAPP1 and TAPP2 genes, uncoupling them from PI(3,4)P2, exhibit increased BCR-induced activation of the kinase Akt and develop lupus-like characteristics including anti-DNA antibodies and deposition of immune complexes in kidneys.

Here, we find that TAPP KI mice develop chronic germinal centers (GCs) with age and show abnormal expression of B-cell activation and memory markers. Upon immunization with T-dependent Ag, TAPP KI mice develop functional but abnormally large GCs, associated with increased GC B-cell survival. Disruption of chronic GCs in TAPP KI mice by deletion of the costimulatory molecule ICOS abrogate anti-DNA and anti-nuclear antibody production in TAPP KI mice, indicating an essential role for GCs.

CKMM protein

30-AC61 100 ug
EUR 325.2
Description: Purified recombinant CKMM protein

CKMM protein

30-1083 1000 units
EUR 949.2
Description: Purified native Human CKMM protein

CKMM protein

30-1084U 100 units
EUR 334.8
Description: Highly pure native Human CKMM protein

CKMM antibody

10-C41A 1 mg
EUR 594
Description: Mouse monoclonal CKMM antibody

CKMM antibody

10-C41B 1 mg
EUR 454.8
Description: Mouse monoclonal CKMM antibody

CKMM antibody

10C-CR3014M1 1 mg
EUR 594
Description: Mouse monoclonal CKMM antibody

CKMM antibody

10C-CR3014M2 1 mg
EUR 454.8
Description: Mouse monoclonal CKMM antibody

CKMM antibody

10C-CR3014M3 1 mg
EUR 594
Description: Mouse monoclonal CKMM antibody

CKMM antibody

10R-8516 100 ul
EUR 471.6
Description: Mouse monoclonal CKMM antibody

CKMM antibody

20C-CR3014G 1 ml
EUR 270
Description: Goat polyclonal CKMM antibody

CKMM antibody

20-CG75 1 ml
EUR 207.6
Description: Goat polyclonal CKMM antibody

CKMM antibody

20-CR75 1 ml
EUR 207.6
Description: Rabbit polyclonal CKMM antibody

CKMM antibody

70-XG47 1 mg
EUR 388.8
Description: Affinity purified Goat polyclonal CKMM antibody

CKMM antibody

70R-15270 100 ug
EUR 392.4
Description: Rabbit polyclonal CKMM antibody

CKMM antibody

70R-33149 100 ug
EUR 522
Description: Rabbit polyclonal CKMM antibody

CKMM antibody

70R-1984 50 ug
EUR 560.4
Description: Rabbit polyclonal CKMM antibody raised against the N terminal of CKM

CKMM antibody

70R-1985 50 ug
EUR 560.4
Description: Rabbit polyclonal CKMM antibody raised against the middle region of CKM

CKMM antibody

20R-CK004 1 L Ask for price
Description: Goat polyclonal CKMM antiserum

CKMM antibody

70R-CG004 1 G Ask for price
Description: Affinity purified Goat polyclonal CKMM antibody

CKMM antibody (HRP)

60R-1648 100 ug
EUR 392.4
Description: Rabbit polyclonal CKMM antibody (HRP)

Rat CKMM Antibody

32939-05111 150 ug
EUR 313.2

CKMM antibody (FITC)

60R-1649 100 ug
EUR 392.4
Description: Rabbit polyclonal CKMM antibody (FITC)

Mouse CKMM Antibody

32938-05111 150 ug
EUR 313.2

CKMM antibody (biotin)

60R-1650 100 ug
EUR 418.8
Description: Rabbit polyclonal CKMM antibody (biotin)

CKMM Blocking Peptide

33R-9549 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CKM antibody, catalog no. 70R-1984

CKMM Blocking Peptide

33R-3630 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CKM antibody, catalog no. 70R-1985

CKMM Type 1 protein

30R-AC026 1 mg
EUR 949.2
Description: Purified recombinant CKMM Type 1 protein

Human ckmm PicoKine ELISA Kit

EK1751 96 wells
EUR 510
Description: For quantitative detection of human ckmm in cell culture supernates, serum and plasma (heparin, EDTA).

Rat CKMM Antibody (Biotin Conjugate)

32939-05121 150 ug
EUR 442.8

Mouse CKMM Antibody (Biotin Conjugate)

32938-05121 150 ug
EUR 442.8

Rat CKMM AssayLite Antibody (APC Conjugate)

32939-05161 150 ug
EUR 513.6

Rat CKMM AssayLite Antibody (FITC Conjugate)

32939-05141 150 ug
EUR 513.6

Rat CKMM AssayLite Antibody (RPE Coinjugate)

32939-05151 150 ug
EUR 513.6

Mouse CKMM AssayLite Antibody (RPE Conjugate)

32938-05151 150 ug
EUR 513.6

Mouse CKMM AssayLite Antibody (APC Conjugate)

32938-05161 150 ug
EUR 513.6

Rat CKMM AssayLite Antibody (PerCP Conjugate)

32939-05171 150 ug
EUR 565.2

Mouse CKMM AssayLite Antibody (FITC Conjugate)

32938-05141 150 ug
EUR 513.6

Recombinant Human CKMM/CKM Protein, His Tag

E40KMH880 20ug
EUR 495

Mouse CKMM AssayLite Antibody (PerCP Conjugate)

32938-05171 150 ug
EUR 565.2

Human Creatine Kinase, Muscle(CKMM) Elisa Kit

EK700626 96 Wells
EUR 0.5

Human Creatine Kinase M-type (CKMM) Antibody

11281-05011 150 ug
EUR 260.4

Recombinant Human Creatine Kinase MM/CKMM Protein

RP02817LQ 50μg
EUR 58.5

Recombinant Human Creatine Kinase, Muscle/CKMM (C-6His)

CC96-10ug 10ug
EUR 169.2
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,10%Glycerol,pH7.5.

Recombinant Human Creatine Kinase, Muscle/CKMM (C-6His)

CC96-1mg 1mg
EUR 2008.8
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,10%Glycerol,pH7.5.

Recombinant Human Creatine Kinase, Muscle/CKMM (C-6His)

CC96-500ug 500ug
EUR 1423.2
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,10%Glycerol,pH7.5.

Recombinant Human Creatine Kinase, Muscle/CKMM (C-6His)

CC96-50ug 50ug
EUR 315.6
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,10%Glycerol,pH7.5.

Recombinant Human Creatine Kinase, Muscle/CKMM (C-6His)

AP75592 1mg
EUR 2369

Human Creatine Kinase M-type (CKMM) Antibody (Biotin Conjugate)

11281-05021 150 ug
EUR 331.2

Human Creatine Kinase M-type (CKMM) AssayLite Antibody (RPE Conjugate)

11281-05051 150 ug
EUR 513.6

Human Creatine Kinase M-type (CKMM) AssayLite Antibody (APC Conjugate)

11281-05061 150 ug
EUR 513.6

Human Creatine Kinase M-type (CKMM) AssayLite Antibody (FITC Conjugate)

11281-05041 150 ug
EUR 513.6

Human Creatine Kinase M-type (CKMM) AssayLite Antibody (PerCP Conjugate)

11281-05071 150 ug
EUR 565.2

anti-CKMM (3C3)

LF-MA0399 100 ul
EUR 400.8
Description: Mouse monoclonal to CKMM

anti-CKMM (11B11)

LF-MA0400 100 ul
EUR 400.8
Description: Mouse monoclonal to CKMM

  Rat CK-MM

1800-2 0.1 mg
EUR 200

  Pig CK-MM

1800-3 0.1 mg
EUR 200

  Mouse CK-MM

1800-1 0.1 mg
EUR 200

Rat CK-MM ELISA

KT-551 96 tests
EUR 595

Pig CK-MM ELISA

KT-552 96 tests
EUR 595

CK-MM Protein

abx060278-100ug 100 ug
EUR 910.8

Rat CK-MM ELISA Kit

ERC0407 96Tests
EUR 625.2

CK-MM Antigen

E64C00703 100ug
EUR 1620

CK-MM Antibody

abx022710-1mg 1 mg
EUR 886.8

CK-MM Antibody

abx022712-1mg 1 mg
EUR 577.2

CK-MM Antibody

abx022713-1ml 1 ml
EUR 444

CK-MM Antibody

abx020743-1mg 1 mg
EUR 577.2

CK-MM Antibody

abx020744-1mg 1 mg
EUR 526.8

Goat CK-MM ELISA Kit

EGTC0407 96Tests
EUR 625.2

CK-MM Type I Protein

abx069790-1mg 1 mg
EUR 886.8

Mouse CK-MM ELISA Kit

EMC0407 96Tests
EUR 625.2

Sheep CK-MM ELISA Kit

ESC0407 96Tests
EUR 625.2

Human CK-MM ELISA Kit

EHC0407 96Tests
EUR 625.2

Human(CK-MM) ELISA Kit

QY-E05359 96T
EUR 433.2

CK-MM Type III Protein

abx069791-1mg 1 mg
EUR 886.8

Monkey CK-MM ELISA Kit

EMKC0407 96Tests
EUR 625.2

Bovine CK-MM ELISA Kit

EBC0407 96Tests
EUR 625.2

Canine CK-MM ELISA Kit

ECC0407 96Tests
EUR 625.2

Porcine CK-MM ELISA Kit

EPC0407 96Tests
EUR 625.2

Nori® Human CK-MM ELISA Kit

GR111506 96-well
EUR 461

Chicken CK-MM ELISA Kit

ECKC0407 96Tests
EUR 625.2

Guinea Pig CK-MM ELISA Kit

EGC0407 96Tests
EUR 625.2

Ck-Mm Antigen Grade Protein

abx060710-1mg 1 mg
EUR 777.6

Human Creatine Kinase MM (CK-MM)

RP-1063 100 ug
EUR 343.2

OAMA01375-1MG - CK-MM Antibody

OAMA01375-1MG 1mg
EUR 359

OAMA01376-1MG - CK-MM Antibody

OAMA01376-1MG 1mg
EUR 299

anti-Creatine kinase-MM(CK-MM) (2C5)

LF-MA0234 100 ul
EUR 400.8
Description: Mouse monoclonal to Creatine kinase-MM(CK-MM)

OPMA04784-1MG - CK-MM Type I Protein

OPMA04784-1MG 1mg
EUR 669

OPMA04785-1MG - CK-MM Type III Protein

OPMA04785-1MG 1mg
EUR 799

Rat Creatine Kinase MM isoenzyme, CK-MM ELISA Kit

CSB-E14405r-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat Creatine Kinase MM isoenzyme, CK-MM in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Creatine Kinase MM isoenzyme, CK-MM ELISA Kit

1-CSB-E14405r
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Creatine Kinase MM isoenzyme, CK-MM in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

YLA1738RA-48T 48T
EUR 465

Rat Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

YLA1738RA-96T 96T
EUR 600

Rat Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

EKC38912-48T 48T
EUR 535.99

Rat Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

EKC38912-5x96T 5x96T
EUR 3637.08

Rat Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

EKC38912-96T 96T
EUR 765.7

Human Creatine Kinase MM isoenzyme(CK-MM) ELISA Kit

CSB-E18038h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Creatine Kinase MM isoenzyme (CK-MM) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Creatine Kinase MM isoenzyme(CK-MM) ELISA Kit

1-CSB-E18038h
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Creatine Kinase MM isoenzyme(CK-MM) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Creatine Kinase MM isoenzyme, CK-MM ELISA Kit

CSB-E14407m-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Mouse Creatine Kinase MM isoenzyme, CK-MM in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Creatine Kinase MM isoenzyme, CK-MM ELISA Kit

1-CSB-E14407m
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse Creatine Kinase MM isoenzyme, CK-MM in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Creatine Kinase MM isoenzyme(CK-MM) ELISA Kit

SL0680Mo -
EUR 528

Human Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

YLA0595HU-48T 48T
EUR 435

Human Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

YLA0595HU-96T 96T
EUR 562.5

Mouse Creatine Kinase MM isoenzyme(CK-MM) Elisa Kit

EK730427 96 Wells
EUR 0.68

Human Creatine Kinase MM isoenzyme(CK-MM) Elisa Kit

EK711916 96 Wells
EUR 0.22

Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

YLA1902MO-48T 48T
EUR 465

Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

YLA1902MO-96T 96T
EUR 600

Human Creatine Kinase MM isoenzyme(CK-MM) ELISA Kit

EKC33304-48T 48T
EUR 597.24

Human Creatine Kinase MM isoenzyme(CK-MM) ELISA Kit

EKC33304-5x96T 5x96T
EUR 4052.7

Human Creatine Kinase MM isoenzyme(CK-MM) ELISA Kit

EKC33304-96T 96T
EUR 853.2

Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

EKC36674-48T 48T
EUR 535.99

Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

EKC36674-5x96T 5x96T
EUR 3637.08

Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

EKC36674-96T 96T
EUR 765.7

Rat Creatine Kinase MM isoEnzyme, CK-MM GENLISA ELISA

KLR1930 1 x 96 wells
EUR 341

Mouse Creatine Kinase MM isoEnzyme, CK-MM GENLISA ELISA

KLM1979 1 x 96 wells
EUR 341

Human Creatine Kinase MM Isoenzyme, CK-MM GENLISA ELISA

KBH3377 1 x 96 wells
EUR 286

Recombinant Human Creatine Kinase MM (CK-MM) Type 3 Isoenzyme

DAG278 1KU
EUR 1095.6

Recombinant Human Creatine Kinase MM (CK-MM) Type I Isoenzyme

DAG281 1mg
EUR 1204.8

OPEF01611-1MG - Recombinant Creatine Kinase MM (CK-MM) Antigen

OPEF01611-1MG 1mg
EUR 792

CKMB Type-2

cki-270 10µg
EUR 60
Description: Recombinant Human Creatine Kinase MB Isoenzyme Type-2

Ckmt2 (GFP-tagged) - Mouse creatine kinase, mitochondrial 2 (Ckmt2)

MG206653 10 µg Ask for price

Moreover, TAPP KI B cells are sufficient to drive chronic GC responses and recapitulate the autoimmune phenotype in BM chimeric mice. Our findings demonstrate a B-cell-intrinsic role of TAPP-PI(3,4)P2 interaction in regulating GC responses and autoantibody production and suggest that uncontrolled Akt activity in B cells can drive autoimmunity.