Heterotrimeric G protein subunits Gαq and Gα11 are inhibited by two cyclic depsipeptides, FR900359 (FR) and YM-254890 (YM), each of that are getting used broadly to implicate Gq/11 proteins within the regulation of various organic processes. An rising main analysis query due to this fact is whether or not the mobile results of each inhibitors are on-target, i.e. mediated through particular inhibition of Gq/11 proteins, or off-target, i.e. the results of nonspecific interactions with different proteins. Right here we introduce a flexible experimental technique to discriminate between these potentialities. We developed a Gαq variant with preserved catalytic exercise, however refractory to FR/YM inhibition. A minimal of two amino acid adjustments have been required and ample to realize full inhibitor resistance. We characterised the
novel mutant in HEK293 cells depleted by CRISPR-Cas9 of endogenous Gαq and Gα11 to make sure exact management over the Gα-dependent mobile signaling route. Utilizing a battery of mobile outcomes with identified and hid Gq contribution, we discovered that FR/YM particularly inhibited mobile alerts following Gαq introduction through transient transfection. Conversely, each inhibitors have been inert throughout all assays in cells expressing the drug-resistant variant. These findings remove the chance that inhibition of non-Gq proteins contributes to the mobile results of the 2 depsipeptides.
We conclude that mixed software of FR or YM together with the drug-resistant Gαq variant is a strong in vitro technique to discern on-target Gq in opposition to off-target non-Gq motion. Consequently, it ought to be of excessive worth for uncovering Gq enter to advanced organic processes with excessive accuracy and the requisite specificity. Signaling between cells can promote each the event and development of most cancers. It has been discovered that chemical and bodily alerts, along with extracellular components, can affect most cancers development.
On this evaluate, we concentrate on the bodily microenvironment of most cancers cells and look at the motion of mechanical, electromagnetic, thermal, and acoustic alerts on most cancers cells, which can present new instructions for most cancers analysis and therapy. Thus, according to a lack of NMDAR operate, cocaine-induced ERK phosphorylation is attenuated. As a result of early NMDAR exercise contributes to the initiation of lasting addiction-relevant neuroadaptations, these information might maintain clues into mobile mechanisms liable for the event of cocaine habit.
Carbon dioxide-dependent sign transduction in mammalian programs
Carbon dioxide (CO2) is a elementary physiological gasoline identified to profoundly affect the behaviour and well being of hundreds of thousands of species inside the plant and animal kingdoms particularly. A latest Royal Society assembly on the subject of ‘Carbon dioxide detection in organic programs’ was extraordinarily revealing when it comes to the multitude of roles that completely different ranges of CO2 play in influencing crops and animals alike. Whereas excellent analysis has been carried out by main researchers within the space of plant biology, neuronal sensing, cell signalling, gasoline transport, irritation, lung operate and medical drugs, there’s nonetheless a lot to be realized about CO2-dependent sensing and signalling.
Notably, whereas a number of key sign transduction pathways and nodes of exercise have been recognized in crops and animals respectively, the exact wiring and sensitivity of those pathways to CO2 stays to be totally elucidated. On this article, we’ll give an summary of the literature referring to CO2-dependent sign transduction in mammalian programs. We’ll spotlight the primary sign transduction hubs by which CO2-dependent signalling is elicited with a view to raised understanding the advanced physiological response to CO2 in mammalian programs.
The principle subjects of dialogue on this article relate to how adjustments in CO2 affect mobile operate by modulation of sign transduction networks influenced by pH, mitochondrial operate, adenylate cyclase, calcium, transcriptional regulators, the adenosine monophosphate-activated protein kinase pathway and direct CO2-dependent protein modifications. Whereas every of those subjects might be mentioned independently, there’s proof of serious cross-talk between these sign transduction pathways as they reply to adjustments in CO2. In contemplating these core hubs of CO2-dependent sign transduction, we hope to delineate widespread components and establish areas wherein future analysis could possibly be greatest directed.
Cocaine-Induced Synaptic Redistribution of NMDARs in Striatal Neurons Alters NMDAR-Dependent Sign Transduction
he consequence of repeated cocaine publicity and extended abstinence on glutamate receptor expression within the nucleus accumbens has been extensively studied. Nevertheless, the early results of cocaine on NMDAR signaling stay unknown. NMDAR signaling is dependent upon the subunit composition, subcellular localization, and the interplay with proteins on the postsynaptic density (PSD), the place NMDARs and different proteins type supercomplexes which might be liable for the signaling pathways activated by NMDAR-induced Ca2+ inflow. Right here, we investigated the impact of cocaine on NMDAR
 Human signal transduction-Smad1 ELISA Kit |
|
CN-03384H1 |
ChemNorm |
96T |
EUR 448 |
Human signal transduction-Smad1 ELISA Kit |
|
CN-03384H2 |
ChemNorm |
48T |
EUR 297 |
 Rat signal transduction-Smad1 ELISA Kit |
|
CN-01425R1 |
ChemNorm |
96T |
EUR 439 |
Rat signal transduction-Smad1 ELISA Kit |
|
CN-01425R2 |
ChemNorm |
48T |
EUR 290 |
 Human signal transduction-Smad7 ELISA Kit |
|
CN-03385H1 |
ChemNorm |
96T |
EUR 448 |
Human signal transduction-Smad7 ELISA Kit |
|
CN-03385H2 |
ChemNorm |
48T |
EUR 297 |
 Rat signal transduction-Smad7 ELISA Kit |
|
CN-01426R1 |
ChemNorm |
96T |
EUR 442 |
Rat signal transduction-Smad7 ELISA Kit |
|
CN-01426R2 |
ChemNorm |
48T |
EUR 293 |
 Human Signal transduction protein CBL- C, CBLC ELISA KIT |
|
ELI-50273h |
Lifescience Market |
96 Tests |
EUR 824 |
 Mouse Signal transduction protein CBL- C, Cblc ELISA KIT |
|
ELI-33118m |
Lifescience Market |
96 Tests |
EUR 865 |
 ELISA Kit) Human Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
RD-Smad1-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 478 |
Human Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
RD-Smad1-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 662 |
 ELISA Kit) Rat Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
RD-Smad1-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 511 |
Rat Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
RD-Smad1-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 709 |
Human Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
DLR-Smad1-Hu-48T |
DL Develop |
48T |
EUR 479 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Mothers Against Decapentaplegic Homolog 1 (Smad1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
DLR-Smad1-Hu-96T |
DL Develop |
96T |
EUR 621 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Mothers Against Decapentaplegic Homolog 1 (Smad1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
DLR-Smad1-Ra-48T |
DL Develop |
48T |
EUR 508 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Mothers Against Decapentaplegic Homolog 1 (Smad1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
DLR-Smad1-Ra-96T |
DL Develop |
96T |
EUR 661 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Mothers Against Decapentaplegic Homolog 1 (Smad1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
RDR-Smad1-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 500 |
Human Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
RDR-Smad1-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 692 |
Rat Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
RDR-Smad1-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 534 |
Rat Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit |
|
RDR-Smad1-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 742 |
 Signal Transduction Monoclonal Antibody) Anti-ERK1 (E32) Signal Transduction Monoclonal Antibody |
|
ABP-MAB-ES001 |
Allele Biotech |
100 ug |
Ask for price |
|
|
 Signal Transduction Monoclonal Antibody) Anti-ERK1 (E19) Signal Transduction Monoclonal Antibody |
|
ABP-MAB-ES002 |
Allele Biotech |
100 ug |
Ask for price |
|
|
 Signal Transduction Monoclonal Antibody) Anti-ERK1/2 (E31R) Signal Transduction Monoclonal Antibody |
|
ABP-MAB-ES003 |
Allele Biotech |
100 ug |
Ask for price |
|
|
 QuantiSir Specific Gene Knockdown Quantification Kit For Signal Transduction - ViDR |
|
P-5008 |
EpiGentek |
96 Assays |
EUR 788.05 |
|
Description: Ask the seller for details |
) Bacillus subtilis Signal transduction histidine-protein kinase/phosphatase DegS (degS) |
|
1-CSB-EP320275BRJ |
Cusabio |
-
EUR 611.00
-
EUR 309.00
-
EUR 1827.00
-
EUR 939.00
-
EUR 1218.00
-
EUR 397.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
|
|
Description: Recombinant Bacillus subtilis Signal transduction histidine-protein kinase/phosphatase DegS(degS) expressed in E.coli |
 Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed |
|
ELISA-1 |
Alpha Diagnostics |
1 |
EUR 202 |
 Sheep Smad1 ELISA Kit |
|
ESS0276 |
Abclonal |
96Tests |
EUR 521 |
 Rat Smad1 ELISA Kit |
|
ERS0276 |
Abclonal |
96Tests |
EUR 521 |
 Rabbit Smad1 ELISA Kit |
|
ERTS0276 |
Abclonal |
96Tests |
EUR 521 |
 Monkey Smad1 ELISA Kit |
|
EMKS0276 |
Abclonal |
96Tests |
EUR 521 |
 Mouse Smad1 ELISA Kit |
|
EMS0276 |
Abclonal |
96Tests |
EUR 521 |
 Porcine Smad1 ELISA Kit |
|
EPS0276 |
Abclonal |
96Tests |
EUR 521 |
 Goat Smad1 ELISA Kit |
|
EGTS0276 |
Abclonal |
96Tests |
EUR 521 |
 Bovine Smad1 ELISA Kit |
|
EBS0276 |
Abclonal |
96Tests |
EUR 521 |
 Chicken Smad1 ELISA Kit |
|
ECKS0276 |
Abclonal |
96Tests |
EUR 521 |
 Canine Smad1 ELISA Kit |
|
ECS0276 |
Abclonal |
96Tests |
EUR 521 |
 Anserini Smad1 ELISA Kit |
|
EAS0276 |
Abclonal |
96Tests |
EUR 521 |
 Smad1 Cell ELISA Kit |
|
abx595549-96tests |
Abbexa |
96 tests |
EUR 637 |
|
|
 Guinea Pig Smad1 ELISA Kit |
|
EGS0276 |
Abclonal |
96Tests |
EUR 521 |
 Smad1 DNA-Binding ELISA Kit |
|
abx596539-96tests |
Abbexa |
96 tests |
EUR 723 |
|
|
 (OKCD06627)) SMAD1 ELISA Kit (Mouse) (OKCD06627) |
|
OKCD06627 |
Aviva Systems Biology |
96 Wells |
EUR 1001 |
|
Description: Description of target: ;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 0.125ng/mL |
 (OKEH05787)) SMAD1 ELISA Kit (Mouse) (OKEH05787) |
|
OKEH05787 |
Aviva Systems Biology |
96 Wells |
EUR 662 |
|
Description: Description of target: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). May play a role in the initiation and maintenance of spermatogenesis. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 31.34 pg/mL |
 (OKEH07161)) SMAD1 ELISA Kit (Rat) (OKEH07161) |
|
OKEH07161 |
Aviva Systems Biology |
96 Wells |
EUR 662 |
|
Description: Description of target: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). Has been shown to be also activated by TGF-beta (transforming growth factor) type I receptor kinase in rat intestinal epithelial cells (IEC 4-1). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.159 ng/mL |
 (OKEH07443)) SMAD1 ELISA Kit (Bovine) (OKEH07443) |
|
OKEH07443 |
Aviva Systems Biology |
96 Wells |
EUR 1092 |
|
Description: Description of target: ;Species reactivity: Bovine;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 36pg/mL |
 (OKWB00419)) SMAD1 ELISA Kit (Rabbit) (OKWB00419) |
|
OKWB00419 |
Aviva Systems Biology |
96 Wells |
EUR 572 |
|
Description: Description of target: ;Species reactivity: Rabbit;Application: ;Assay info: Assay Type: Quantitative Sandwich ELISA;Sensitivity: 0.188 ng/mL |
) Recombinant KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) |
|
4-RPL650Hu01 |
Cloud-Clone |
-
EUR 499.62
-
EUR 236.00
-
EUR 1598.56
-
EUR 599.52
-
EUR 1099.04
-
EUR 397.00
-
EUR 3846.40
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
|
|
Description: Recombinant Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 expressed in: E.coli |
Recombinant KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) |
|
4-RPL650Mu01 |
Cloud-Clone |
-
EUR 510.37
-
EUR 239.00
-
EUR 1638.88
-
EUR 612.96
-
EUR 1125.92
-
EUR 404.00
-
EUR 3947.20
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
|
|
Description: Recombinant Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 expressed in: E.coli |
 Antibody) KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Antibody |
|
20-abx128380 |
Abbexa |
-
EUR 453.00
-
EUR 133.00
-
EUR 1302.00
-
EUR 620.00
-
EUR 342.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
|
 Antibody) KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 2 (KHDRBS2) Antibody |
|
20-abx307678 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
|
KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Antibody |
|
20-abx104762 |
Abbexa |
-
EUR 467.00
-
EUR 133.00
-
EUR 1344.00
-
EUR 634.00
-
EUR 342.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
|
KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 2 (KHDRBS2) Antibody |
|
abx034990-400ul |
Abbexa |
400 ul |
EUR 523 |
|
|
KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 2 (KHDRBS2) Antibody |
|
abx034990-80l |
Abbexa |
80 µl |
EUR 286 |
|
|
KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 2 (KHDRBS2) Antibody |
|
20-abx007227 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
|
KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 2 (KHDRBS2) Antibody |
|
abx224066-100ug |
Abbexa |
100 ug |
EUR 411 |
|
|
KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Antibody |
|
20-abx327286 |
Abbexa |
|
|
|
|
) ViraDuctin Retrovirus Transduction Kit (40 Transductions) |
|
RV-200 |
Cell Biolabs |
40 transductions |
EUR 363 |
|
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells. |
) ViraDuctin Retrovirus Transduction Kit (200 Transductions) |
|
RV-201 |
Cell Biolabs |
200 transductions |
EUR 780 |
|
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells. |
) ViraDuctin Lentivirus Transduction Kit (40 Transductions) |
|
LTV-200 |
Cell Biolabs |
40 transductions |
EUR 345 |
|
Description: Lentiviral expression has many advantages over other viruses, including the ability to infect both proliferating and non-proliferating cells. The efficiency of lentivirus infection of target cells, however, tends to be lower. Additives such as Polybrene can increase transduction efficiencies, but even then only a small fraction of lentiviral vectors can trasduce many target cell lines. Our ViraDuctin Lentivirus Transduction Kit provides superior lentiviral transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene. This system is ideal for many primary cells as well as immobilized cells. |
) ViraDuctin Lentivirus Transduction Kit (200 Transductions) |
|
LTV-201 |
Cell Biolabs |
200 transductions |
EUR 751 |
|
Description: Lentiviral expression has many advantages over other viruses, including the ability to infect both proliferating and non-proliferating cells. The efficiency of lentivirus infection of target cells, however, tends to be lower. Additives such as Polybrene can increase transduction efficiencies, but even then only a small fraction of lentiviral vectors can trasduce many target cell lines. Our ViraDuctin Lentivirus Transduction Kit provides superior lentiviral transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene. This system is ideal for many primary cells as well as immobilized cells. |
) ViraDuctin AAV Transduction Kit (10 Transductions) |
|
AAV-200 |
Cell Biolabs |
10 transductions |
EUR 421 |
|
Description: The process by which adeno-associated virus (AAV) infects host cells includes viral binding and entry, intracellular trafficking, nuclear transport, and viral second strand DNA synthesis. The second strand DNA synthesis has been shown to be the rate limiting step, which leads to inefficient transduction by AAV vectors. ViraDuctin AAV Transduction Kit is a proprietary, multi-reagent formulation designed to increase the efficiency of transduction by AAV vectors. Reagent preparation takes only 10 minutes prior to host cell infection. |
) ViraDuctin AAV Transduction Kit (50 Transductions) |
|
AAV-201 |
Cell Biolabs |
50 transductions |
EUR 972 |
|
Description: The process by which adeno-associated virus (AAV) infects host cells includes viral binding and entry, intracellular trafficking, nuclear transport, and viral second strand DNA synthesis. The second strand DNA synthesis has been shown to be the rate limiting step, which leads to inefficient transduction by AAV vectors. ViraDuctin AAV Transduction Kit is a proprietary, multi-reagent formulation designed to increase the efficiency of transduction by AAV vectors. Reagent preparation takes only 10 minutes prior to host cell infection. |
) ViraDuctin Adenovirus Transduction Kit (10 Transductions) |
|
AD-200 |
Cell Biolabs |
10 transductions |
EUR 415 |
|
Description: Adenoviral infection of most cells is mediated largely by the coxsackievirus-adenovirus receptor (CAR). In many cells, however, this receptor is either absent or present at extremely low levels, reducing the efficiency of adenovirus uptake. ViraDuctin Adenovirus Transduction Reagent is designed specifically to increase the efficiency of adenoviral transduction without regard to the level of CAR expression on the surface of the target cells. |
) ViraDuctin Adenovirus Transduction Kit (50 Transductions) |
|
AD-201 |
Cell Biolabs |
50 transductions |
EUR 949 |
|
Description: Adenoviral infection of most cells is mediated largely by the coxsackievirus-adenovirus receptor (CAR). In many cells, however, this receptor is either absent or present at extremely low levels, reducing the efficiency of adenovirus uptake. ViraDuctin Adenovirus Transduction Reagent is designed specifically to increase the efficiency of adenoviral transduction without regard to the level of CAR expression on the surface of the target cells. |
 Protein) Mouse KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Protein |
|
20-abx067639 |
Abbexa |
-
EUR 718.00
-
EUR 286.00
-
EUR 2207.00
-
EUR 843.00
-
EUR 509.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
|
 Protein) Human KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1) Protein |
|
20-abx166241 |
Abbexa |
-
EUR 704.00
-
EUR 286.00
-
EUR 2151.00
-
EUR 829.00
-
EUR 495.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
|
 Smad1 Colorimetric Cell-Based ELISA Kit |
|
EKC1526 |
BosterBio |
100ul |
EUR 572 |
 DNA Binding ELISA Kit) Smad1 (pS187) DNA Binding ELISA Kit |
|
abx596638-96tests |
Abbexa |
96 tests |
EUR 754 |
|
|
 Smad1 / 5 / 9 Cell ELISA Kit |
|
abx595866-96tests |
Abbexa |
96 tests |
EUR 637 |
|
|
) Smad1 DNA-Binding ELISA Kit (OKAG00440) |
|
OKAG00440 |
Aviva Systems Biology |
96 Wells |
EUR 753 |
|
Description: Description of target: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.;Species reactivity: Human, Mouse, Rat;Application: ELISA;Assay info: Assay Type: Qualitative DNA Binding ELISA ;Sensitivity: |
 SMAD1 Antibody |
|
CSB-PA998747- |
Cusabio |
|
EUR 335 |
|
|
|
Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
SMAD1 Antibody |
|
CSB-PA998747-100ul |
Cusabio |
100ul |
EUR 316 |
|
|
|
Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
SMAD1 Antibody |
|
CSB-PA825094- |
Cusabio |
|
EUR 335 |
|
|
|
Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
SMAD1 Antibody |
|
CSB-PA825094-100ul |
Cusabio |
100ul |
EUR 316 |
|
|
|
Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
SMAD1 Antibody |
|
1-CSB-PA841004 |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200 |
SMAD1 Antibody |
|
1-CSB-PA009928 |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human, Mouse, Rat, Monkey. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000 |
SMAD1 Antibody |
|
1-CSB-PA004104 |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/10000 |
SMAD1 Antibody |
|
1-CSB-PA005538 |
Cusabio |
|
|
|
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Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000 |
SMAD1 Antibody |
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1-CSB-PA042170 |
Cusabio |
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Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100 |
SMAD1 Antibody |
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1-CSB-PA021786GA01HU |
Cusabio |
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Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
SMAD1 Antibody |
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1-CSB-PA618998HA01HU |
Cusabio |
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Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:500 |
SMAD1 Antibody |
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1-CSB-PA618998LA01HU |
Cusabio |
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Description: A polyclonal antibody against SMAD1. Recognizes SMAD1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:600, IF:1:50-1:200 |
SMAD1 Antibody |
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32160-100ul |
SAB |
100ul |
EUR 252 |
 SMAD1 siRNA |
|
20-abx934282 |
Abbexa |
|
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SMAD1 siRNA |
|
20-abx934283 |
Abbexa |
|
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|
|
 Smad1 Antibody |
|
48784-100ul |
SAB |
100ul |
EUR 333 |
Smad1 Antibody |
|
48784-50ul |
SAB |
50ul |
EUR 239 |
Smad1 Antibody |
|
49149-100ul |
SAB |
100ul |
EUR 333 |
Smad1 Antibody |
|
49149-50ul |
SAB |
50ul |
EUR 239 |
Smad1 Antibody |
|
AF6451 |
Affbiotech |
200ul |
EUR 304 |
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Description: Smad1 Antibody detects endogenous levels of total Smad1. |
subunit composition and subcellular localization after each intraperitoneal non-contingent cocaine and response-contingent intravenous cocaine self-administration in mice. We discovered that repeated cocaine publicity, whatever the route or contingency of drug administration, decreases NMDAR interactions with the PSD and synaptic lipid rafts within the accumbens shell and dorsal striatum. We offer proof that cocaine triggers an early redistribution of NMDARs from synaptic to extrasynaptic websites, and that this adaptation has implications within the activation of downstream signaling pathways.
